Graphene Oxide Nanofluidic Ion Channels with Two-Gene Rolling Circle Amplification for Ultrasensitive and Specific Detection of SARS-CoV-2

Lin, M.H.; Yang, M.Y.; Xiao, Y.L.; Zhao, J.; Shang, Z.W.; Liu, X.Y.; Wang, L.P.; Pan, J.; Yi, X.Q.; Zhang, X.J.*; Xia, F. Graphene Oxide Nanofluidic Ion Channels with Two-Gene Rolling Circle Amplification for Ultrasensitive and Specific Detection of SARS-CoV-2. Analytical Chemistry. 2025 Oct 14;97(40):22153-22163. 新冠病毒实现快速、灵敏、特异性检测是早期诊断与疫情防控的关键难题。论文联用双基因 RCA 与氧化石墨烯修饰纳米孔道，依靠 DNA 纳米花调控离子整流信号，双靶标协同实现极低拷贝假病毒检出，灵敏度较单基因提升 10 倍。